Journal: Heliyon
Article Title: Identification and experimental verification of immune-related hub genes in intervertebral disc degeneration
doi: 10.1016/j.heliyon.2024.e34530
Figure Lengend Snippet: In vitro validation experiment. (A) Identification of M1 macrophages. (B) Cell coculture of nucleus pulposus cells and macrophages. (C) IL-1β and TNF-α levels in the supernatant after 24 h of coculture. (D) Levels of NR1H3, SORT1, PTGDS, AGT, IRF1, and TGFB2 mRNA in nucleus pulposus cells after 24 h of coculture. (E) Levels of NR1H3, SORT1, PTGDS, AGT, IRF1, and TGFB2 protein in nucleus pulposus cells after 24 h of coculture. The unedited images are referenced in . *: P < 0.05, **: P < 0.01, ***: P < 0.010, ****: P < 0.0001, ns: P > 0.05.
Article Snippet: The following antibodies were added and incubated at 4 °C overnight: NR1H3 antibody (14351-1-AP, proteintech, 1:2000), SORT1 antibody (12369-1-AP, proteintech, 1:2000), PTGDS antibody (10754-2-AP, proteintech, 1:1000), AGT antibody (11992-1-AP, proteintech, 1:1000), IRF1 antibody (11335-1-AP, proteintech, 1:500), TGFB2 antibody (28426-1-AP, proteintech, 1:500), and GAPDH antibody (10494-1-AP, proteintech, 1:5000).
Techniques: In Vitro, Biomarker Discovery